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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Flying Syringes for Emerging Enzootic Virus Screening: Proof of Concept for the Development of Noninvasive Xenosurveillance Tools Based on Tsetse Flies
Transboundary and Emerging Diseases, Volume 2023, Article 9145289, Year 2023
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Description
Pathogen transfers between wild and domestic animals and between animals and humans are increasing. Their dramatic consequences for public and veterinary health as well as for conservation call for innovative and user-friendly methods for pathogen surveillance in wildlife. Xenosurveillance, a method based on the use of invertebrates (e.g., mosquitoes, hematophagous flies, leeches, cadaveric arthropods) to sample animal tissues (e.g., blood) and the associated pathogens, is one of these tools. Previously, we demonstrated that hematophagous flies, such as tsetse flies, could be useful to detect and identify the etiological agents of malaria in a diverse range of mammals in Gabon. However, we did not assess whether this method can be also used to detect viruses. In the present study, we experimentally fed tsetse flies (Glossina fuscipes fuscipes) rabbit blood containing different viruses of medical or veterinary importance (Zika, Dengue, Chikungunya, African swine fever, Bluetongue, and peste des petits ruminants viruses). Then, we used quantitative PCR (i) to determine for how long viral nucleic acid fragments remained detectable in the tsetse midgut during blood digestion and (ii) to compare two blood meal preservation methods (i.e., FTA cards and RNAlater solution) tested using tsetse flies engorged with blood and dengue-2 virus. All viruses remained detectable for 6 days after feeding, although the detection probability significantly decreased over time. FTA cards and RNAlater solution gave similar results in terms of virus detection. Our results demonstrate that xenosurveillance using blood-engorged tsetse flies is a valuable tool to track and survey viruses in wildlife in Sub-Saharan Africa. © 2023 Adeline Valente et al.
Authors & Co-Authors
Jiolle, Davy
France, Montpellier
Université de Montpellier
Ravel, Sophie
France, Montpellier
Interactions Hôtes-vecteurs-parasites-environnement Dans Les Maladies Tropicales Négligées Dues Aux Trypanosomatides Intertryp
Porciani, Angélique
France, Montpellier
Université de Montpellier
Vial, Laurence
France, Paris
Inrae
Michaud, Vincent
France, Paris
Inrae
Kwiatek, Olivier
France, Paris
Inrae
Pédarrieu, Aurélie
France, Paris
Inrae
Missé, Dorothée A.
France, Montpellier
Université de Montpellier
Ferraris, Pauline
France, Montpellier
Université de Montpellier
Bretagnolle, François
France, Paris
Cnrs Centre National de la Recherche Scientifique
Bitome-Essono, Paul Yannick
Gabon, Libreville
Institut de Recherche en Ecologie Tropicale
Makanga, Boris Kevin
Gabon, Libreville
Institut de Recherche en Ecologie Tropicale
Rougeron, Virginie
South Africa, Gqeberha
Nelson Mandela University
Prugnolle, Franck
France, Montpellier
Université de Montpellier
South Africa, Gqeberha
Nelson Mandela University
Paupy, Christophe
France, Montpellier
Université de Montpellier
Statistics
Citations: 1
Authors: 15
Affiliations: 6
Identifiers
Doi:
10.1155/2023/9145289
ISSN:
18651674
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Gabon