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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Design of immunogenic peptides from Mycobacterium tuberculosis genes expressed during macrophage infection
Tuberculosis, Volume 89, No. 3, Year 2009
Notification
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Description
In vitro diagnosis of MTB-infection uses MTB-proteins coded for by genes of the region of differentiation 1 (RD1) of the MTB genome. This study wants to test if proteins preferentially expressed during MTB-intracellular growth might provide new targets for the diagnosis of MTB-infection. To this end seventy-five multiepitopic HLA-promiscuous MTB-peptides were designed by quantitative implemented peptide-binding motif analysis from 3 MTB-protein genes expressed in activated human macrophages (MA), 4 genes expressed during growth in non-activated human macrophages (MN-A), 12 housekeeping genes (HKG) and 6 genes of the RD1 region (RD1) as control. ELISpot for IFN-was performed to measure the responses of PBMCs deriving from 45 patients affected by active tuberculosis and 34 controls. In active-TB patients, the mean response to RD1-derived peptides was higher than that to either MA (p < 0.01), MN-A (p < 0.008) or HKG (p < 0.01) derived peptides. In TST-positive subjects all selected peptides elicited significant IFN-T-cell responses (p < 0.02 compared to TST-negatives), but without differences between the subgroups. Further, T-cell responses to RD1 peptides were lower in the 23 active-TB treated patients than in the untreated ones (p < 0.01). The response to MA peptides in treated active-TB was higher than when untreated (p < 0.01). These results demonstrate that the use of in vitro models of MTB-intracellular infection to select MTB gene products for further in silico and in vitro assessment of their immunogenicity have the potential to identify novel antigens amenable to the design of new tools for diagnosis and monitoring of tuberculosis. © 2009.
Authors & Co-Authors
Seghrouchni, Fouad
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Contini, Silvia
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Markova, Roumiana
Bulgaria, Sofia
National Center of Infectious and Parasitic Diseases Bulgaria
Drenska, Roumiana
Bulgaria, Sofia
National Center of Infectious and Parasitic Diseases Bulgaria
Sadki, Khalid
Morocco, Agdal Rabat
Institut National D'hygiène
Baassi, Larbii
Morocco, Agdal Rabat
Institut National D'hygiène
Todorova, Yana
Bulgaria, Sofia
National Center of Infectious and Parasitic Diseases Bulgaria
Terzieva, Velislava
Bulgaria, Sofia
National Center of Infectious and Parasitic Diseases Bulgaria
Bocchino, Marialuisa
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Cappelli, Giulia
Italy, Rome
Consiglio Nazionale Delle Ricerche
Altieri, Alfonso Maria
Italy, Rome
Azienda Ospedaliera San Camillo Forlanini
Alma, Mario Giuseppe
Italy, Rome
Azienda Ospedaliera San Camillo Forlanini
Benjouad, Abdelaziz
Morocco, Rabat
Mohammed V University in Rabat
Mariani, F.
Italy, Rome
Consiglio Nazionale Delle Ricerche
Petrunov, Bogdan
Bulgaria, Sofia
National Center of Infectious and Parasitic Diseases Bulgaria
Colizzi, Vittorio C.
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
El Aouad, Rajae
Morocco, Agdal Rabat
Institut National D'hygiène
Saltini, Cesare
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Amicosante, Massimo
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Statistics
Citations: 19
Authors: 19
Affiliations: 6
Identifiers
Doi:
10.1016/j.tube.2009.03.005
ISSN:
14729792
Research Areas
Genetics And Genomics
Infectious Diseases
Study Approach
Quantitative