Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Genotype determination in Moroccan hepatitis B chronic carriers
Infection, Genetics and Evolution, Volume 8, No. 3, Year 2008
Notification
URL copied to clipboard!
Description
Background: In Morocco, chronic liver disease related to hepatitis B virus (HBV) is a public health burden. Treatment of chronic hepatitis B is often complicated by the appearance of escape mutants after treatment with nucleoside analogs, especially with genotypes responsible for the more severe form of the disease. Objectives: In the present study we investigate the prevalence of the different HBV genotypes in Morocco since no previous careful study has been attempted. Methods: Epidemiological data from 91 chronically infected patients (45 women and 46 men) were collected prospectively. Sera were tested for anti-HBc IgG, HBeAg, anti-HBe antibody and liver enzymes. Restriction Fragment Length Polymorphism (RFLP) analysis was confirmed by subsequent sequencing of the pre-S and S region of the viral genome in order to determine which HBV genotypes were prevalent among Moroccan patients. Results: The mean age was 41 ± 12.4 years. Ten patients (11%) were positive for hepatitis B e antigen (HBeAg) and 81 (89%) were positive for anti-HBe antibodies. By the RLFP method, genotype D, pattern D2, was found in the 77 cases where HBV was successfully amplified. Phylogenetic analysis based on pre-S/S sequences revealed that genotype D in Morocco differed from others D strains subgenotypes (D1, D2, D3 and D4). In addition, the pre-core mutant defined as HBeAg-negative/anti-HBe-positive and HBV DNA positive was detected in 86% of cases. Conclusions: Our results clearly show that genotype D and pre-core mutant are highly prevalent in Morocco. © 2008 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Ezzikouri, Sayeh
Morocco, Casablanca
Institut Pasteur du Maroc
Chemin, Isabelle A.
France, Paris
Inserm
Chafik, Abdelaziz
Morocco, El Jadida
Faculté Des Sciences D'el Jadida
Wakrim, Lahcen
Morocco, Casablanca
Institut Pasteur du Maroc
Nourlil, Jalal
Morocco, Casablanca
Institut Pasteur du Maroc
Malki, Abdelouhab El
Morocco, Casablanca
Institut Pasteur du Maroc
Marchio, Agnès
France, Paris
Institut Pasteur, Paris
Dejean, Anne
France, Paris
Institut Pasteur, Paris
Hassar, Mohammed
Morocco, Casablanca
Institut Pasteur du Maroc
Trépo, Christian R.
France, Paris
Inserm
Pineau, Pascal
France, Paris
Institut Pasteur, Paris
Benjelloun, Soumaya
Morocco, Casablanca
Institut Pasteur du Maroc
Statistics
Citations: 34
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1016/j.meegid.2008.01.010
ISSN:
15671348
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Morocco
Participants Gender
Male
Female