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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNA
Cell, Volume 47, No. 1, Year 1986
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Description
Closed-circular HBV DNA was introduced into cells of the established human hepatoma culture HepG2. The culture medium of one of 40 single-cell clones contained HBV surface antigen (HBsAg), core-related antigens ( HBc eAg), and HBV DNA sequences. HBV DNA and DNA polymerase activity were detected in particles resembling both nucleocapsids and complete virions (Dane particles). Intracellular integrated and extrachromosomal HBV DNA sequences were detected. Relaxed-circular and single-stranded forms of viral DNA were identified as likely replicative intermediates of the HBV genome. In conclusion, in vitro production of Dane-like particles by transformed human hepatocytes has been achieved. This model should be valuable as a cell culture system for studying virus replication and virus-host cell interactions. © 1986.
Authors & Co-Authors
Sureau, Camille
United States, Boston
Harvard T.h. Chan School of Public Health
Romet-Lemonne, Jean Loup
United States, Boston
Harvard T.h. Chan School of Public Health
France, Tours
Hopital Bretonneau
Mullins, James I.
United States, Boston
Harvard T.h. Chan School of Public Health
Essex, Max E.
United States, Boston
Harvard T.h. Chan School of Public Health
Statistics
Citations: 342
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/0092-8674(86)90364-8
ISSN:
00928674
Research Areas
Genetics And Genomics
Infectious Diseases