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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Genetic variation of the dihydrofolate reductase gene in Plasmodium vivax in Snoul, northeastern Cambodia
Acta Tropica, Volume 92, No. 1, Year 2004
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Description
In Plasmodium vivax, pyrimethamine resistance is associated with amino acid substitutions Ser117Asn and Ser58Arg in dihydrofolate reductase (DHFR), which correspond to Ser108Asn and Cys59Arg in the Plasmodium falciparum homolog, respectively. Sequence variations within the DHFR domain of 32 P. vivax isolates from Snoul, Cambodia, were analyzed by direct sequencing of polymerase chain reaction (PCR) products. Sequence polymorphisms within the entire DHFR domain were limited to codons 58 and 117 and GGDN tandem repeat units. A large majority (30 of 32) of isolates were characterized to be double mutants (Arg-58 and Asn-117) and associated with the presence of two GGDN repeat units. Only one isolate was of wild-type with three GGDN repeat units, and an additional isolate was of mixed type. Our data suggest that most Cambodian P. vivax isolates display double dhfr mutations associated with pyrimethamine resistance, as in the neighboring countries in Southeast Asia. Further molecular characterization of P. vivax isolates from different endemic areas may be a useful alternative approach to establish the epidemiology of drug-resistant malaria. © 2004 Published by Elsevier B.V.
Authors & Co-Authors
de Pécoulas, Philippe Eldin
France, Caen
Université de Caen Normandie
Tahar, Rachida
France, Paris
Institut Pasteur, Paris
Yi, Poravuth
Cambodia, Phnom Penh
National Centre for Parasitology
Thai, Khieng Heng
Cambodia, Phnom Penh
National Centre for Parasitology
Basco, Leonardo K.
Cameroon, Yaounde
Laboratoire de Recherche Sur le Paludisme Yaounde
Statistics
Citations: 25
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/j.actatropica.2004.03.011
ISSN:
0001706X
Research Areas
Genetics And Genomics
Infectious Diseases