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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Strategies for manipulating metabolic fluxes in biotechnology
Bioorganic Chemistry, Volume 23, No. 4, Year 1995
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Description
Strategies for biotechnologically manipulating metabolic fluxes are critically examined in relation to a model system. The common idea of first identifying the rate-limiting enzyme in the biosynthetic pathway to a desired end-product, and then increasing its activity, is shown to be completely ineffective; such manipulation typically produces only trivial changes in flux. Manipulating the activities of all of the enzymes in a biosynthetic pathway by amounts calculated to increase a desired flux while leaving all other fluxes and all concentrations unchanged is potentially effective, and can be applied to any system without regard to its regulatory design. however, it requires accurate knowledge of the initial state of the system and the ability to make precise changes to numerous activities. The classical information about the regulatory mechanisms that exist in living organisms suggests that one can make much simpler manipulations, involving only the steps that remove the desired end-product, with almost equally satisfactory results. © 1995 by Academic Press, Inc.
Authors & Co-Authors
Cornish-Bowden, Athel J.
France, Paris
Cnrs Centre National de la Recherche Scientifique
Hofmeyr, J. H.S.
South Africa, Stellenbosch
Stellenbosch University
Cárdenas, María Luz
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 91
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1006/bioo.1995.1030
ISSN:
00452068
Research Areas
Noncommunicable Diseases