Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Role of Pycnoporus coccineus laccase in the degradation of aromatic compounds in olive oil mill wastewater
Enzyme and Microbial Technology, Volume 36, No. 4, Year 2005
Notification
URL copied to clipboard!
Description
In a previous work was reported the ability of Pycnoporus coccineus to decolorize olive oil mill wastewaters (OOMW) without an additional carbon source. We studied the composition of the enzymatic system involved in the process. The fungus secreted only laccase under the different culture conditions studied even in presence of compounds promoting the production of peroxidases. The highest laccase levels were attained in Cu2+-ethanol medium (100,000 U/l after 45 incubation-days). A single isoenzyme was purified with a yield of 79%. This laccase is a glycoprotein (8% N-linked carbohydrate) with a molecular mass and pI of 61.5 kDa and 3.7, respectively. The highest oxidation rate was obtained around pH 3.5 for ABTS and DMP and the highest DMP oxidation at 60°C. The enzyme was stable at pH 7 at room temperature and showed a half-life of 8 and 2 h at 50 and 60°C, respectively. The treatment of OOMW with the laccase showed similar results to those reported with the fungus indicating that laccase plays an important role in the degradative process. The high levels of laccase secreted by P. coccineus and its stability suggest that it could be a useful tool for this and other environmental applications. © 2004 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Jaouani, Atef
Belgium, Brussels
Les Amis Des Instituts Pasteur à Bruxelles
Pennînckx, Michel J.
Belgium, Brussels
Les Amis Des Instituts Pasteur à Bruxelles
Martínez, Ángel T.
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Martínez, María Jesús
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Statistics
Citations: 146
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1016/j.enzmictec.2004.11.011
ISSN:
01410229
Research Areas
Environmental