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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Adaptive HIV-Specific B Cell-Derived Humoral Immune Defenses of the Intestinal Mucosa in Children Exposed to HIV via Breast-Feeding
PLoS ONE, Volume 8, No. 5, Article e63408, Year 2013
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Description
Background:We evaluated whether B cell-derived immune defenses of the gastro-intestinal tract are activated to produce HIV-specific antibodies in children continuously exposed to HIV via breast-feeding.Methods:Couples of HIV-1-infected mothers (n = 14) and their breastfed non HIV-infected (n = 8) and HIV-infected (n = 6) babies, and healthy HIV-negative mothers and breastfed babies (n = 10) as controls, were prospectively included at the Complexe Pédiatrique of Bangui, Central African Republic. Immunoglobulins (IgA, IgG and IgM) and anti-gp160 antibodies from mother's milk and stools of breastfed children were quantified by ELISA. Immunoaffinity purified anti-gp160 antibodies were characterized functionally regarding their capacity to reduce attachment and/or infection of R5- and X4- tropic HIV-1 strains on human colorectal epithelial HT29 cells line or monocyte-derived-macrophages (MDM).Results:The levels of total IgA and IgG were increased in milk of HIV-infected mothers and stools of HIV-exposed children, indicating the activation of B cell-derived mucosal immunity. Breast milk samples as well as stool samples from HIV-negative and HIV-infected babies exposed to HIV by breast-feeding, contained high levels of HIV-specific antibodies, mainly IgG antibodies, less frequently IgA antibodies, and rarely IgM antibodies. Relative ratios of excretion by reference to lactoferrin calculated for HIV-specific IgA, IgG and IgM in stools of HIV-exposed children were largely superior to 1, indicating active production of HIV-specific antibodies by the intestinal mucosa. Antibodies to gp160 purified from pooled stools of HIV-exposed breastfed children inhibited the attachment of HIV-1NDK on HT29 cells by 63% and on MDM by 77%, and the attachment of HIV-1JRCSF on MDM by 40%; and the infection of MDM by HIV-1JRCSF by 93%.Conclusions:The intestinal mucosa of children exposed to HIV by breast-feeding produces HIV-specific antibodies harbouring in vitro major functional properties against HIV. These observations lay the conceptual basis for the design of a prophylactic vaccine against HIV in exposed children. © 2013 Moussa et al.
Authors & Co-Authors
Moussa, Sandrine
Central African Republic, Bangui
Institut Pasteur de Bangui
Jenabian, Mohammad Ali
France, Paris
Hôpital Européen Georges-pompidou
Canada, Montreal
Mcgill University Health Centre, Montreal Chest Institute
Gody, Jean Chrysostome
Central African Republic
Faculté Des Sciences de la Santé de Bangui
Léal, Josiane
Central African Republic, Bangui
Institut Pasteur de Bangui
Grésenguet, Gérard
Central African Republic
Faculté Des Sciences de la Santé de Bangui
Le Faou, Alain E.
Central African Republic, Bangui
Institut Pasteur de Bangui
France, Vandoeouvre-les-nancy
Cibles Thérapeutiques, Formulation et Expertise Préclinique du Médicament
Bélec, Laurent
France, Paris
Hôpital Européen Georges-pompidou
France, Paris
Université Paris Cité
Statistics
Citations: 7
Authors: 7
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0063408
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Study Locations
Central African Republic