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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemistry
Performance characteristics of qPCR assays targeting human- and ruminant-associated bacteroidetes for microbial source tracking across sixteen countries on six continents
Environmental Science and Technology, Volume 47, No. 15, Year 2013
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Description
Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle-, or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and nontarget samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant-targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable, suggesting potential for improvements in human-targeted methods. © 2013 American Chemical Society.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3737603/bin/es304367t_si_001.pdf
Authors & Co-Authors
Reischer, Georg H.
Austria, Vienna
Technische Universität Wien
Austria, Vienna
Interuniversity Cooperation Centre Water and Health
Ebdon, James
United Kingdom, Brighton
University of Brighton
Bauer, Johanna M.
Austria, Vienna
Technische Universität Wien
Schuster, Nathalie
Austria, Vienna
Technische Universität Wien
Ahmed, Warish
Australia, Melbourne
Csiro Land and Water
As̊tröm, Johan
Sweden, Gothenburg
Chalmers University of Technology
Blanch, Anicet R.
Spain, Barcelona
Universitat de Barcelona
Blöschl, Günter
Austria, Vienna
Technische Universität Wien
Byamukama, Denis
Uganda, Kampala
Makerere University
Coakley, Tricia
United States, Lexington
University of Kentucky
Ferguson, Christobel
Australia, Sydney
Als Water Sciences Group
Goshu, Goraw
Ethiopia, Bahir Dar
Bahir Dar University
Ko, Gwangpyo
South Korea, Seoul
Seoul National University
de Roda-Husman, Ana Maria
Netherlands, Bilthoven
Rijksinstituut Voor Volksgezondheid en Milieu
Mushi, Douglas
Tanzania
Sokoine University
Poma, Ramiro
Argentina, Salta
Universidad Nacional de Salta
Pradhan, Bandana
Nepal, Kathmandu
Tribhuvan University
Rajal, Veronica
Argentina, Salta
Universidad Nacional de Salta
Schade, Margit A.
Germany, Munich
Bavarian Environment Agency
Sommer, Regina
Austria, Vienna
Interuniversity Cooperation Centre Water and Health
Austria, Vienna
Medizinische Universitat Wien, Zentrum Für Pathophysiologie, Infektiologie Und Immunologie
Taylor, Huw
United Kingdom, Brighton
University of Brighton
Töth, Erika M.
Hungary, Budapest
Eötvös Loránd Tudományegyetem
Vrajmasu, Virgil
Romania, Tulcea
Veterinary State Laboratory
Wuertz, Stefan
United States, Davis
University of California, Davis
Mach, Robert Ludwig
Austria, Vienna
Technische Universität Wien
Farnleitner, Andreas H.
Austria, Vienna
Technische Universität Wien
Austria, Vienna
Interuniversity Cooperation Centre Water and Health
Statistics
Citations: 106
Authors: 26
Affiliations: 20
Identifiers
Doi:
10.1021/es304367t
ISSN:
0013936X
e-ISSN:
15205851
Study Approach
Quantitative