Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Targeted and persistent effects of NO mediated by S-nitrosation of tissue thiols in arteries with endothelial dysfunction
Nitric Oxide - Biology and Chemistry, Volume 17, No. 1, Year 2007
Notification
URL copied to clipboard!
Description
We have previously demonstrated that in endothelium-denuded arteries, S-nitrosation of cysteine residues is a mechanism of formation of releasable nitric oxide (NO) stores, accounting for the long-lasting relaxation induced by S-nitrosating agents like S-nitrosoglutathione (GSNO). Here, we have investigated whether such effects could also be obtained in arteries exhibiting oxidative stress-associated endothelial dysfunction. Rats were implanted or not with a minipump delivering saline or angiotensin II for 14 days. As expected, aorta from angiotensin II-infused rats exhibited increased level of superoxide anions (as evaluated with dihydroethidine as fluorescent probe) and a reduced relaxation to acetylcholine in comparison to saline group. Unlike aortic rings with endothelium from controls, those from angiotensin II-infused rats exhibited persistent hyporesponsiveness to phenylephrine after pre-exposure to GSNO, as well as relaxation upon addition of N-acetylcysteine (NAC, which can displace NO from cysteine-NO residues) or HgCl2 (which cleaves S-NO bonds). In aorta from angiotensin II-infused rats, GSNO also induced a persistent increase in cysteine-NO residues (as determined using anti-cysteine-NO antiserum), which was blunted by NAC and HgCl2. These data indicate that (i) the vasorelaxant influence of releasable NO stores is unmasked by endothelial dysfunction (ii) S-nitrosation of cysteine residues remains an effective mechanism of formation of releasable NO stores in arteries exhibited oxidative stress-associated endothelial dysfunction. Thus, formation of releasable NO stores by S-nitrosating agents allows targeted vasculoprotective effects of NO at sites of endothelial dysfunction. © 2007 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Sarr, Mamadou Moustapha
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Pessac
Centre de Recherche Cardio-thoracique de Bordeaux
France, Paris
École Pratique Des Hautes Études
Chataigneau, Marta
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Paris
École Pratique Des Hautes Études
Étienne-Selloum, Nelly
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Paris
École Pratique Des Hautes Études
Diallo, Aminata Sall
France, Pessac
Centre de Recherche Cardio-thoracique de Bordeaux
France, Paris
École Pratique Des Hautes Études
Schott, Christa
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Paris
École Pratique Des Hautes Études
Geffard, Michael R.
Senegal, Dakar
Universite Cheikh Anta Diop Faculte de Medecine et de Pharmacie
France, Paris
École Pratique Des Hautes Études
Stoclet, Jean Claude
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Paris
École Pratique Des Hautes Études
Schini-Kerth, Valérie Barbara
France, Paris
Cnrs Centre National de la Recherche Scientifique
France, Paris
École Pratique Des Hautes Études
Müller, Bernard
France, Bordeaux
Université de Bordeaux
France, Pessac
Centre de Recherche Cardio-thoracique de Bordeaux
France, Paris
École Pratique Des Hautes Études
Statistics
Citations: 9
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.niox.2007.04.003
ISSN:
10898603