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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of cytomegalovirus DNA on dried blood spots collected from infants infected with HIV: An in-house method adaptable in resource-limited settings
Journal of Virological Methods, Volume 193, No. 2, Year 2013
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Description
In countries with limited resources, infants infected with HIV are highly exposed to CMV co-infection which probably represents a major risk factor for disease progression in this population. This study aimed to evaluate the performance of a low cost CMV DNA extraction method from DBS and the feasibility of its implementation in laboratories of 4 countries with limited resources. DNA was extracted from DBS with a cationic resin (chelex 100) and amplified with an "in house" real time CMV PCR. Dilutions of a quantified whole blood sample were spotted on paper to evaluate the 95% detection limit. A DBS quality control panel was analyzed in all laboratories. CMV PCR was compared between DBS and liquid whole blood (gold standard) in 2 populations: 418 transplanted patients and 59 infants infected with HIV (median age of 2 months). The CMV PCR 95% detection limit in DBS was 3.87log10copies/mL. Its positive and negative predictive values for CMV diagnosis in infants infected with HIV were 100% and 87.5% respectively. Quality control panels gave consistent qualitative results in all laboratories. This assay had high predictive values for CMV diagnosis in infants infected with HIV and its implementation in resource-limited countries with limited resources is feasible. © 2013 Elsevier B.V.
Authors & Co-Authors
Leruez-Ville, Marianne
France, Paris
Hôpital Necker Enfants Malades
Ngin, Sopheak
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Guilleminot, Tiffany
France, Paris
Hôpital Necker Enfants Malades
Kfutwah, Anfumbom Kitu Womeyi
Cameroon, Yaounde
Pasteur Centre of Cameroon
Moussa, Sandrine
Central African Republic, Bangui
Institut Pasteur de Bangui
Tran, Ton L.
Viet Nam, Ho Chi Minh City
Pasteur Institute in ho Chi Minh City
Nerrienet, Eric
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Statistics
Citations: 7
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.jviromet.2013.07.024
ISSN:
01660934
e-ISSN:
18790984
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Qualitative