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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Technical Aspects and Clinical Limitations of Sperm DNA Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations
World Journal of Men's Health, Volume 41, Year 2023
Notification
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Description
Purpose: Sperm DNA fragmentation (SDF) is a functional sperm abnormality that can impact reproductive potential, for which four assays have been described in the recently published sixth edition of the WHO laboratory manual for the examination and processing of human semen. The purpose of this study was to examine the global practices related to the use of SDF assays and investigate the barriers and limitations that clinicians face in incorporating these tests into their practice. Materials and Methods: Clinicians managing male infertility were invited to complete an online survey on practices related to SDF diagnostic and treatment approaches. Their responses related to the technical aspects of SDF testing, current professional society guidelines, and the literature were used to generate expert recommendations via the Delphi method. Finally, challenges related to SDF that the clinicians encounter in their daily practice were captured. Results: The survey was completed by 436 reproductive clinicians. Overall, terminal deoxynucleotidyl transferase deoxyuridine triphosphate Nick-End Labeling (TUNEL) is the most commonly used assay chosen by 28.6%, followed by the sperm chromatin structure assay (24.1%), and the sperm chromatin dispersion (19.1%). The choice of the assay was largely influenced by availability (70% of respondents). A threshold of 30% was the most selected cut-off value for elevated SDF by 33.7% of clinicians. Of respondents, 53.6% recommend SDF testing after 3 to 5 days of abstinence. Although 75.3% believe SDF testing can provide an explanation for many unknown causes of infertility, the main limiting factors selected by respondents are a lack of professional society guideline recommendations (62.7%) and an absence of globally accepted references for SDF interpretation (50.3%). Conclusions: This study represents the largest global survey on the technical aspects of SDF testing as well as the barriers encountered by clinicians. Unified global recommendations regarding clinician implementation and standard laboratory interpretation of SDF testing are crucial. Copyright © 2023 Korean Society for Sexual Medicine and Andrology.
Authors & Co-Authors
Agarwal, Ashok K.
United States, Cleveland
Cleveland Clinic Foundation
Farkouh, Ala'A N.
Unknown Affiliation
Saleh, Ramadan Abdou
Egypt, Sohag
Sohag University
Hamoda, Taha Abo Almagd Abdel Meguid
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Minya
Minia University
Salvio, Gianmaria
Italy, Ancona
Università Politecnica Delle Marche
Boitrelle, Florence
France, Poissy
Centre Hospitalier Intercommunal Poissy-st-germain-en-laye
France, Jouy-en-josas
Unité Biologie de la Reproduction, Environnement, Epigénétique et Développement
Harraz, Ahmed M.
Egypt, Mansoura
Mansoura University, Urology and Nephrology Center
Kuwait, Safat
Farwania Hospital
Kuwait, Kuwait City
Sabah al Ahmad Urology Center
Abou Ghayda, Ramy
United States, Cleveland
Case Western Reserve University
Kavoussi, Parviz Keikhosrow
Unknown Affiliation
Gul, Murat
Unknown Affiliation
Toprak, Tuncay
Turkey, Istanbul
University of Health Sciences
Russo, Giorgio Ivan
Italy, Catania
Università Degli Studi Di Catania
Durairajanayagam, Damayanthi
Malaysia, Shah Alam
Universiti Teknologi Mara
Rambhatla, Amarnath
United States, Detroit
Henry Ford Health System
Birowo, Ponco
Indonesia, Depok
Universitas Indonesia
Cannarella, Rossella
Italy, Catania
Università Degli Studi Di Catania
United States, Cleveland
Cleveland Clinic Foundation
Phuoc, Nguyen Ho Vinh
Unknown Affiliation
Zini, Armand S.
Canada, Montreal
Université Mcgill
Arafa, Mohamed Mostafa
Qatar, Doha
Hamad Medical Corporation
Qatar, Doha
Weill Cornell Medicine-qatar
Egypt, Giza
Cairo University
Wyns, Christine
Belgium, Louvain-la-neuve
Université Catholique de Louvain
Tremellen, Kelton P.
Australia, Adelaide
Flinders University
Sarikaya, Selcuk
Turkey, Istanbul
University of Health Sciences
Lewis, Sheena E.M.
United Kingdom, Belfast
Examenlab Ltd.
Evenson, Donald P.
Unknown Affiliation
Ko, Edmund Yuey Kun
United States, Loma Linda
Loma Linda University Health
Calogero, Aldo E.
Kuwait, Safat
Farwania Hospital
Bahar, Fahmi
Unknown Affiliation
Martínez, Marlon P.
Philippines, Manila
University of Santo Tomas Hospital
Ambar, Rafael Favero
Brazil, Santo Andre
Institute Ideia Fértil of Reproductive Health
Colpi, Giovannimaria
Switzerland, Lugano
Next Fertility Procrea
Bakircioǧlu, Mustafa Emre
Unknown Affiliation
Henkel, Ralf Reinhold
United Kingdom, London
Imperial College London
South Africa, Bellville
University of the Western Cape
Kandil, Hussein
Unknown Affiliation
Serefoglu, E. C.
Turkey, Istanbul
Biruni Üniversitesi
Tsujimura, Akira
Japan, Urayasu
Juntendo Urayasu Hospital
Marino, Angelo
Unknown Affiliation
Adamyan, Aram
Unknown Affiliation
Žilaitiené, Birute
Lithuania, Kaunas
Lietuvos Sveikatos Mokslų Universitetas
Ozer, Cevahir
Turkey, Ankara
Başkent Üniversitesi
Pescatori, Edoardo Stefano
Unknown Affiliation
Vogiatzi, Paraskevi
Unknown Affiliation
Busetto, Gian Maria
Italy, Foggia
Università Degli Studi Di Foggia
Balercia, Giancarlo
Italy, Ancona
Università Politecnica Delle Marche
Akhavizadegan, Hamed
Iran, Tehran
Tehran University of Medical Sciences
Sajadi, Hesamoddin
Iran, Tehran
Iranian Academic Center for Education, Culture and Research
Taniguchi, Hisanori
Japan, Hirakata
Kansai Medical University
Park, Hyunjun
South Korea, Yangsan
Medical School of Pusan National University
South Korea, Busan
Pusan National University Hospital
Rosas, Israel Maldonado
Unknown Affiliation
Al-Marhoon, Mohammed Salim
Oman, Muscat
Sultan Qaboos University
Pinggera, Germar Michael
Austria, Innsbruck
Medizinische Universitat Innsbruck
Kothari, Priyank
Unknown Affiliation
Mićić, Sava R.
Unknown Affiliation
Homa, Sheryl T.
United Kingdom, Canterbury
University of Kent
Long, Tran Quang Tien
Viet Nam, Hanoi
Hanoi Obstetrics and Gynecology Hospital
Zohdy, Wael Ahmed
Egypt, Giza
Cairo University
Atmoko, Widi
Indonesia, Depok
Universitas Indonesia
Ibrahim, Wael
Malaysia, Subang Jaya
Taylor's University Malaysia
Sabbaghian, Marjan
Iran, Tehran
Iranian Academic Center for Education, Culture and Research
Abumelha, Saad Mohammed
Saudi Arabia, Riyadh
King Abdulaziz Medical City - Riyadh
Chung, Eric
Australia, Woolloongabba
Princess Alexandra Hospital
Ugur, Muhammet Rasit
Unknown Affiliation
Selim, Osama Mohamed
Egypt, Giza
Cairo University
Darbandi, Mahsa
Iran, Tehran
Hope Generation Foundation
Jamali, Mounir
Morocco, Agdal Rabat
Military Hospital Mohammed V
de la Rosette, Jean Jmch
Turkey, Istanbul
İstanbul Medipol Üniversitesi
Kuroda, Shinnosuke No Suke
United States, Cleveland
Cleveland Clinic Foundation
Smith, Ryan P.
United States, Charlottesville
University of Virginia School of Medicine
Başer, Aykut
Turkey, Bandirma
Bandırma Onyedi Eylül University
Kalkanlı, Arif
Turkey, Istanbul
T. C. Sağlık Bakanlığı, Taksim Eğitim ve Araştirma Hastanesi
Tadros, Nicholas N.
United States, Carbondale
Southern Illinois University Carbondale
Aydos, Kaan
Turkey, Ankara
Ankara Üniversitesi
Mierzwa, Tiago César
Unknown Affiliation
Khalafalla, Kareim
Qatar, Doha
Hamad Medical Corporation
United States, Houston
University of Texas Medical School at Houston
United States, Houston
The University of Texas Md Anderson Cancer Center
Malhotra, Vineet
Unknown Affiliation
Moussa, Mohamad Ahmad
Lebanon, Beirut
Université Libanaise
Finocchi, Federica
Italy, Ancona
Università Politecnica Delle Marche
Giulioni, Carlo
Italy, Ancona
Università Politecnica Delle Marche
Çeker, Gökhan
Unknown Affiliation
Zenoaga-Barbăroșie, Cătălina
Romania, Bucharest
Universitatea Din Bucuresti
Barrett, Trenton L.
Unknown Affiliation
Kadıoğlu, Ateş
Turkey, Istanbul
Istanbul Üniversitesi
Jindal, Sunil K.
India, Meerut
Jindal Hospital
Böcü, Kadir
Unknown Affiliation
Karthikeyan, Vilvapathy Senguttuvan
India, Chennai
Apollo Hospitals Group
Franco, Giorgio
Italy, Rome
Azienda Ospedaliera Universitaria Policlinico Umberto I
Garrido, Nicolás
Spain, Valencia
Instituto de Investigación Sanitaria la fe
Gherabi, Nazim
Algeria, Algiers
Université D’alger 1
Sokolakis, Ioannis
Greece, Thessaloniki
Aristotle University of Thessaloniki
Palani, Ayad F.
Iraq, Kalar City
University of Garmian
Çalik, Gökhan
Turkey, Istanbul
İstanbul Medipol Üniversitesi
Kulaksiz, Deniz
Turkey, Istanbul
University of Health Sciences
Simopoulou, Mara C.
Greece, Athens
National and Kapodistrian University of Athens
Dokuzeylül Güngör, Nur
Turkey, Istanbul
Bahçeşehir Üniversitesi
Blecher, Gideon Adam
Australia, Clayton
Monash University
Falcone, Marco
Italy, Turin
Azienda Sanitaria Ospedaliera Molinette San Giovanni Battista Di Torino
Preto, Mirko
Italy, Turin
Azienda Sanitaria Ospedaliera Molinette San Giovanni Battista Di Torino
Amar, Édouard T.
France, Neuilly-sur-seine
Hôpital Américain de Paris
Le, Tan V.
Unknown Affiliation
Ahn, Sun-tae
South Korea, Seoul
Korea University College of Medicine
Rocco, L.
Italy, Naples
Università Degli Studi Della Campania Luigi Vanvitelli
Savira, Missy
Indonesia, Depok
Universitas Indonesia
Rajmil, Osvaldo
Spain, Barcelona
Fundacio Puigvert
Darbandi, Sara
Iran, Tehran
Hope Generation Foundation
Söǧütdelen, Emrullah
Turkey, Bolu
Bolu Abant İzzet Baysal Üniversitesi
Boeri, Luca
Italy, Milan
Fondazione Irccs Ca' Granda Ospedale Maggiore Policlinico
Hakim, Lukman
Indonesia, Surabaya
Universitas Airlangga
Morimoto, Yoshiharu
Unknown Affiliation
Sofikitis, Nikolaos V.
Greece, Ioannina
Faculty of Medicine
Mahmutoglu, Asli Metin
Turkey, Yozgat
Bozok Üniversitesi
Al-Hashimi, Manaf
United Arab Emirates, Abu Dhabi
Burjeel Hospital
Ziouziou, Imad
Morocco, Massa
Souss-massa National Park
Anagnostopoulou, Christina
Unknown Affiliation
Lin, Haocheng
China, Beijing
Peking University Third Hospital
Shah, Rupin
Unknown Affiliation
Statistics
Citations: 2
Authors: 114
Affiliations: 92
Identifiers
Doi:
10.5534/wjmh.230076
ISSN:
22874208
Research Areas
Genetics And Genomics
Health System And Policy
Sexual And Reproductive Health
Study Design
Cross Sectional Study
Study Approach
Quantitative
Participants Gender
Male