Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children
PLoS ONE, Volume 9, No. 2, Article e86448, Year 2014
Notification
URL copied to clipboard!
Description
Background: Advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. We aimed to determine the prevalence and clinical characteristics of human bocavirus (hBoV), human rhinovirus (hRV), polyomavirus-WU (WUPyV) and -KI (KIPyV) and human coronaviruses (CoV)-OC43, -NL63, -HKU1 and -229E among children hospitalized with lower respiratory tract infections (LRTI). Methods: Multiplex real-time reverse-transcriptase polymerase chain reaction was undertaken on archived nasopharyngeal aspirates from HIV-infected and -uninfected children (<2 years age) hospitalized for LRTI, who had been previously investigated for respiratory syncytial virus, human metapneumovirus, parainfluenza I-III, adenovirus and influenza A/B. Results: At least one of these viruses were identified in 274 (53.0%) of 517 and in 509 (54.0%) of 943 LRTI-episodes in HIV-infected and -uninfected children, respectively. Human rhinovirus was the most prevalent in HIV-infected (31.7%) and - uninfected children (32.0%), followed by CoV-OC43 (12.2%) and hBoV (9.5%) in HIV-infected; and by hBoV (13.3%) and WUPyV (11.9%) in HIV-uninfected children. Polyomavirus-KI (8.9% vs. 4.8%; p = 0.002) and CoV-OC43 (12.2% vs. 3.6%; p< 0.001) were more prevalent in HIV-infected than -uninfected children. Combined with previously-tested viruses, respiratory viruses were identified in 60.9% of HIV-infected and 78.3% of HIV-uninfected children. The newly tested viruses were detected at high frequency in association with other respiratory viruses, including previously-investigated viruses (22.8% in HIV-infected and 28.5% in HIV-uninfected children). Conclusions: We established that combined with previously-investigated viruses, at least one respiratory virus was identified in the majority of HIV-infected and HIV-uninfected children hospitalized for LRTI. The high frequency of viral coinfections illustrates the complexities in attributing causality to specific viruses in the aetiology of LRTI and may indicate a synergetic role of viral co-infections in the pathogenesis of childhood LRTI. © 2014 Nunes et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3911925/bin/pone.0086448.s001.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3911925/bin/pone.0086448.s002.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3911925/bin/pone.0086448.s003.docx
Authors & Co-Authors
Nunes, Marta C.
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
Kuschner, Zachary
United States, Stony Brook
Renaissance School of Medicine at Stony Brook University
Rabede, Zelda
South Africa, Johannesburg
University of the Witwatersrand
Madimabe, Richard
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
van Niekerk, A. B.N.
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
Moloi, Jackie
South Africa, Johannesburg
Molecular and Immunology Division
Kuwanda, Locadiah
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
Rossen, John Wilhelmus A.
Netherlands, Groningen
Universitair Medisch Centrum Groningen
Klugman, K. P.
South Africa, Johannesburg
University of the Witwatersrand
United States, Atlanta
Rollins School of Public Health
Adrian, Peter V.
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
Madhi, Shabir A.
South Africa, Pretoria
National Research Foundation
South Africa, Johannesburg
University of the Witwatersrand
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 46
Authors: 11
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0086448
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study