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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Adenovirus detection in shellfish and urban sewage in Morocco (Casablanca region) by the polymerase chain reaction
Journal of Virological Methods, Volume 126, No. 1-2, Year 2005
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Description
Human enteric viruses are largely excreted in faeces. These resistance of these viruses in the environment makes their faecal-oral transmission easier. Filter feeder organisms such as the mussels are bio-accumulators of viruses contaminating their aquatic environment. Thus, undercooked shellfish consumption involves sanitary risks. Thirty samples of mussels (Mytilus sp.), were tested, half were from an aquaculture origin, the others were from an area more exposed to faecal pollution. Fifteen sewage samples from this last area were also examined. Viruses were extracted from the digestive tissue by direct elution method in a glycine/NaCl pH 9.5 buffer followed by PEG 8000 precipitation. The PEG pellets were used for DNA extraction by proteinase K and phenol/chloroform. The molecular characterization, by PCR using specific adenovirus primers revealed that shellfish growing on Mohammedia (a town in the Casablanca outskirts) littoral are contaminated whereas those chosen from aquaculture and bought in the central market were not contaminated. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Karamoko, Yahaya
Morocco, Casablanca
Hassan Ii University of Casablanca
Morocco, Tetouan
Faculté Des Sciences de Tétouan
Ibenyassine, Khadija
Morocco, Casablanca
Hassan Ii University of Casablanca
AitMhand, Rajaa
Morocco, Casablanca
Hassan Ii University of Casablanca
Idaomar, Mouhamed
Morocco, Tetouan
Faculté Des Sciences de Tétouan
Ennaji, M. M.
Morocco, Casablanca
Hassan Ii University of Casablanca
Statistics
Citations: 36
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.jviromet.2005.02.003
ISSN:
01660934
Research Areas
Environmental
Genetics And Genomics
Health System And Policy
Study Locations
Morocco