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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Eco-geographical diversity of cowpea bradyrhizobia in Senegal is marked by dominance of two genetic types
Systematic and Applied Microbiology, Volume 37, No. 2, Year 2014
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Description
The genetic diversity of native cowpea rhizobia originating from 60 sites across four eco-geographic zones in Senegal was studied. More than 300 cowpea nodules were analyzed by PCR-RFLP of the 16S-23S rDNA InterGenic Spacer region (IGS). Alignments of IGS sequences indicated that all genotypes were grouping within the Bradyrhizobium genus. The geographical distribution showed that apart from five IGS types, the others were specifically found in only one region. The diversity was significantly higher in the Senegal River valley zone, which presents lower mean annual rainfalls and slightly alkaline soils. Interestingly, two IGS types dominated the Senegalese rhizobial collection, one IGS type (VI) was found on more than half of the nodules collected in the northern Senegal River valley while another IGS type (I) was recovered from the great majority of nodules in the three other regions sampled. Two representative strains from each of these two dominant types were isolated and further analyzed. Multi Locus Sequence Analyses using 6 housekeeping genes indicate that they belong to a new Bradyrhizobium species closely related to B. yuanmingense. Phylogenetic analyses of 2 symbiotic genes nodC and nifH show that they are clustered with B. arachidis. Physiological tests on these strains have shown that under laboratory conditions, the growth of the IGS type VI strains was slightly less affected by a higher osmotic strength in the medium and to alkaline pH, which corroborates the soil physico-chemical parameters. © 2013 Elsevier GmbH.
Authors & Co-Authors
Wade, Tatiana Krasova
Senegal, Dakar
Centre Ird de Bel Air
Le Quéré, Antoine J.L.
France, Montpellier
Inrae's Occitanie-montpellier Centre
Laguerre, Gisèle
France, Montpellier
Inrae's Occitanie-montpellier Centre
N'Zoué, Angèle
France, Montpellier
Inrae's Occitanie-montpellier Centre
N’Dione, Jacques André
Senegal, Dakar
Third World Forum Dakar
doRego, Francis
Senegal, Dakar
Centre Ird de Bel Air
Sadio, Oumar
France, Plouzane
Laboratoire Des Sciences de L'environnement Marin Lemar
Ndoye, Ibrahima
Senegal, Dakar
Centre Ird de Bel Air
Neyra, Marc
Senegal, Dakar
Centre Ird de Bel Air
France, Montpellier
Inrae's Occitanie-montpellier Centre
France, Villeurbanne
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Statistics
Citations: 29
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.syapm.2013.10.002
e-ISSN:
16180984
Research Areas
Genetics And Genomics
Study Locations
Senegal