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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Characterization of bacteriocin ST8KF produced by a kefir isolate Lactobacillus plantarum ST8KF
International Dairy Journal, Volume 17, No. 3, Year 2007
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Description
Lactobacillus plantarum ST8KF, isolated from kefir, produced a 3.5 kDa bacteriocin (bacST8KF) active against Lb. casei, Lb. salivarius, Lb. curvatus and Listeria innocua. BacST8KF was sensitive to proteolytic enzymes, but stable between pH 2.0 and 10.0, and heat resistant (20 min at 121 °C). BacST8KF did not adsorb to the surface of the producer cell. Maximum activity (25,600 AU mL-1) was recorded in MRS broth with glucose, in MRS broth with glucose replaced by sucrose, and in MRS broth with glucose, supplemented with KH2PO4 after 24 h at 30 °C. Tri-ammonium citrate and glycerol in excess of 5.0 g L-1 repressed bacST8KF production. Production of bacST8KF increased from 800 AU mL-1 after 3 h of fermentation in MRS broth at 30 °C to 12,800 AU mL-1 after 9 h and to 51,200 AU mL-1 after 27 h. These results suggest that bacST8KF may be a secondary metabolite and shows that its mode of activity is bacteriostatic. © 2006 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Powell, J. E.
South Africa, Stellenbosch
Stellenbosch University
Witthuhn, R. C.
South Africa, Stellenbosch
Stellenbosch University
Todorov, S. D.
South Africa, Stellenbosch
Stellenbosch University
Dicks, Leon Milner Theodore
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 156
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1016/j.idairyj.2006.02.012
ISSN:
09586946